概述

  • 产品名称Anti-AMD1抗体
    参阅全部 AMD1 一抗
  • 描述
    兔多克隆抗体to AMD1
  • 经测试应用适用于: ICC/IF, WB, IHC-P, IHC-Fr, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    A synthetic peptide corresponding to a sequence at the C terminus of human AMD1, identical to the related mouse and rat sequence.

  • 阳性对照
    • IHC-P: Mammary cancer sections WB: Rat kidney tissue lysis

性能

应用

Our Abpromise guarantee covers the use of ab65820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 38 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
ICC Use a concentration of 2 - 3 µg/ml.

靶标

  • 通路Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.
  • 序列相似性Belongs to the eukaryotic AdoMetDC family.
  • 翻译后修饰Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adenosylmethionine decarboxylase 1 antibody
    • AdoMetDC antibody
    • AMD1 antibody
    • DCAM_HUMAN antibody
    • EC 4.1.1.50 antibody
    • FLJ26964 antibody
    • S adenosylmethionine decarboxylase 1 antibody
    • S adenosylmethionine decarboxylase proenzyme antibody
    • S-adenosylmethionine decarboxylase beta chain antibody
    • SAMDC antibody
    see all

Anti-AMD1 antibody 图像

  • ab65820, at 0.5µg/ml, staining AMD1 in the cytoplasm of formalin fixed and paraffin embedded mammary cancer tissue sections.
  • Anti-AMD1 antibody (ab65820) at 1 µg/ml + Rat kidney tissue lysis

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65820 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65820 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-AMD1 antibody (ab65820)参考文献

This product has been referenced in:
  • Dos Santos Ramos R  et al. Regulation of the polyamine metabolic pathway in the endometrium of cows during early diestrus. Mol Reprod Dev N/A:N/A (2014). Cow . Read more (PubMed: 24659573) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab65820.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"