Anti-AMBRA1抗体(ab69501)

概述

  • 产品名称Anti-AMBRA1抗体
    参阅全部 AMBRA1 一抗
  • 描述
    兔多克隆抗体to AMBRA1
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 15 amino acid peptide from near the carboxy terminus of human AMBRA1

  • 阳性对照
    • 3T3 (NIH) cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab69501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 143 kDa (predicted molecular weight: 143 kDa).

靶标

  • 功能Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.
  • 序列相似性Contains 3 WD repeats.
  • 细胞定位Cytoplasmic vesicle > autophagosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating molecule in beclin 1 regulated autophagy antibody
    • Activating molecule in BECN1 regulated autophagy protein 1 antibody
    • Activating molecule in BECN1-regulated autophagy protein 1 antibody
    • Ambra1 antibody
    • AMRA1_HUMAN antibody
    • FLJ20294 antibody
    • Hypothetical protein FLJ20294 antibody
    • Hypothetical protein LOC55626 antibody
    • KIAA1736 antibody
    • MGC33725 antibody
    • WDR94 antibody
    see all

Anti-AMBRA1 antibody 图像

  • Anti-AMBRA1 antibody (ab69501) at 1 µg/ml + 3T3 cell lysate at 15 µg

    Secondary
    Anti-rabbit

    Predicted band size : 143 kDa
    Observed band size : 143 kDa
  • ab69501 at 5 µg/ml staining Ambra1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Immunofluorescence of Ambra1 in Human Brain cells using ab69501 at 20 ug/ml.

Anti-AMBRA1 antibody (ab69501)参考文献

ab69501 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"