Anti-AMBRA1抗体(ab59141)

概述

  • 产品名称Anti-AMBRA1抗体
    参阅全部 AMBRA1 一抗
  • 描述
    兔多克隆抗体to AMBRA1
  • 特异性This antibody reacts specifically with 120kDa of mouse protein.
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from the N terminal region of mouse AMBRA1.

性能

应用

Our Abpromise guarantee covers the use of ab59141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 120 kDa (predicted molecular weight: 139 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/100 - 1/500.

靶标

  • 功能Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation.
  • 序列相似性Contains 3 WD repeats.
  • 细胞定位Cytoplasmic vesicle > autophagosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating molecule in beclin 1 regulated autophagy antibody
    • Activating molecule in BECN1 regulated autophagy protein 1 antibody
    • Activating molecule in BECN1-regulated autophagy protein 1 antibody
    • Ambra1 antibody
    • AMRA1_HUMAN antibody
    • FLJ20294 antibody
    • Hypothetical protein FLJ20294 antibody
    • Hypothetical protein LOC55626 antibody
    • KIAA1736 antibody
    • MGC33725 antibody
    • WDR94 antibody
    see all

Anti-AMBRA1 antibody 图像

  • IHC image of ab59141 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59141, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-AMBRA1 antibody (ab59141)参考文献

ab59141 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"