概述

  • 产品名称Anti-AMBN抗体
    参阅全部 AMBN 一抗
  • 描述
    小鼠多克隆抗体to AMBN
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    AMBN (NP_057603, 1 a.a. ~ 447 a.a) full-length human protein.

  • 阳性对照
    • AMBN transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab72776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).

靶标

  • 功能Involved in the mineralization and structural organization of enamel.
  • 组织特异性Ameloblast-specific. Located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel.
  • 序列相似性Belongs to the ameloblastin family.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMBN antibody
    • AMBN_HUMAN antibody
    • Ameloblastin (enamel matrix protein) antibody
    • Ameloblastin antibody

Anti-AMBN antibody 图像

  • All lanes : Anti-AMBN antibody (ab72776) at 1/500 dilution

    Lane 1 : AMBN transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-AMBN antibody (ab72776)参考文献

ab72776 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer
Sample Mouse Tissue sections (Incisor)
Specification Incisor
Permeabilization No
Fixative Paraformaldehyde
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提交于 Jan 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"