Anti-AMBN抗体(ab116347)

概述

  • 产品名称Anti-AMBN抗体
    参阅全部 AMBN 一抗
  • 描述
    兔多克隆抗体to AMBN
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Guinea pig, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 286-335 (RPGFEGMPHN PAMGGDFTLE FDSPVAATKG PENEEGGAQG SPMPEANPDN) of Human AMBN (NP_057603).

  • 阳性对照
    • Jurkat cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab116347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P 1/400. Antigen retrieval is not essential but may optimise staining.

靶标

  • 功能Involved in the mineralization and structural organization of enamel.
  • 组织特异性Ameloblast-specific. Located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel.
  • 序列相似性Belongs to the ameloblastin family.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMBN antibody
    • AMBN_HUMAN antibody
    • Ameloblastin (enamel matrix protein) antibody
    • Ameloblastin antibody

Anti-AMBN antibody 图像

  • ab116347 staining AMBN in Human Tooth tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/400) for 24 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-AMBN antibody (ab116347) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 48 kDa
    Gel concentration: 12%.

Anti-AMBN antibody (ab116347)参考文献

ab116347 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (Tooth)
Specification Tooth
Permeabilization No
Fixative Formaldehyde
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提交于 Dec 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"