概述

  • 产品名称Anti-Aly抗体[11G5]
    参阅全部 Aly 一抗
  • 描述
    小鼠单克隆抗体[11G5] to Aly
  • 经测试应用适用于: Flow Cyt, IHC-P, ICC/IF, ELISA, WB, IP, Electron Microscopymore details
  • 种属反应性
    与反应: Mouse, Human, Xenopus laevis
  • 免疫原

    Recombinant human ALY/ REF fusion protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purified from supernatant.
  • 克隆单克隆
  • 克隆编号11G5
  • 骨髓瘤Sp2/0
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50. PubMed: 17103222
ELISA Use at an assay dependent concentration.
WB 1/1500. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
IP Use at an assay dependent concentration. PubMed: 17103222
Electron Microscopy Use at an assay dependent concentration. PubMed: 22261716

靶标

  • 功能Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between THOC4 and the THO complex. TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. THOC4 in conjunction with THOC5 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA.
    Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Acts as chaperone and promotes the dimerization of transcription factors containing basic leucine zipper (bZIP) domains and thereby promotes transcriptional activation. Plays a role in mRNA processing and export. May function as scaffold that mediates interactions between proteins and/or RNA. Is part of the exon junction complex that remains associated with spliced mRNA and plays an important role in mRNA export and nonsense-mediated RNA decay. Directs mRNA derived from Herpes simplex virus intron-less genes to the NXF1-mediated export pathway.
  • 序列相似性Belongs to the THOC4 family.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Arg-50 and Arg-204 are dimethylated, probably to asymmetric dimethylarginine.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Nucleus speckle. Cytoplasm. Colocalizes with the core EJC, THOC4, NXF1 and DDX39B in the nucleus and nuclear speckles. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ally of AML-1 and LEF-1 antibody
    • Ally of AML1 and LEF1 antibody
    • ALY antibody
    • ALY/REF antibody
    • Aly/REF export factor antibody
    • BEF antibody
    • bZIP enhancing factor antibody
    • bZIP-enhancing factor BEF antibody
    • REF antibody
    • THO complex 4 antibody
    • THO complex subunit 4 antibody
    • Tho4 antibody
    • thoc4 antibody
    • THOC4_HUMAN antibody
    • Transcriptional coactivator Aly/REF antibody
    • Transcriptional coactivator antibody
    see all

Anti-Aly antibody [11G5] 图像

  • ICC/IF image of ab6141 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6141, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab6141 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6141, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HeLa cells stained with ab6141 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6141, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Aly antibody [11G5] (ab6141)参考文献

This product has been referenced in:
  • Katahira J  et al. Human TREX component Thoc5 affects alternative polyadenylation site choice by recruiting mammalian cleavage factor I. Nucleic Acids Res 41:7060-7072 (2013). IP ; Human . Read more (PubMed: 23685434) »
  • Ding D  et al. The role of deimination in ATP5b mRNA transport in a transgenic mouse model of multiple sclerosis. EMBO Rep 13:230-6 (2012). WB, Electron Microscopy ; Mouse . Read more (PubMed: 22261716) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HCT116)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 50 µg
Specification HCT116
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Nov 30 2016

Thank you for your enquiry. Unfortunately, the originator will not give me any more information regarding the use of this antibody in IF. I have removed this application from the datasheet since I cannot obtain any further information. I apologi...

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