概述

  • 产品名称Anti-ALX3抗体
    参阅全部 ALX3 一抗
  • 描述
    兔多克隆抗体to ALX3
  • 特异性Detects endogenous levels of total ALX3 protein.
  • 经测试应用适用于: WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide from internal of human ALX3.

  • 阳性对照
    • 293 cell extract and HeLa cells

性能

应用

Our Abpromise guarantee covers the use of ab64985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

靶标

  • 相关性ALX3 (aristaless-like homeobox 3) is a nuclear protein with a homeobox DNA-binding domain. It functions as a transcriptional regulator with a possible role in patterning of the mesoderm during development. Preferential methylation of the ALX3 promoter is associated with advanced-stage neuroblastoma tumors.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • ALX 3 antibody
    • ALX3 homeobox 3 antibody
    • Aristaless like homeobox 3 antibody
    • FND1 antibody
    • Homeobox protein aristaless like 3 antibody
    • MGC138212 antibody
    • MGC141988 antibody
    • Proline rich transcription factor ALX3 antibody
    see all

Anti-ALX3 antibody 图像

  • All lanes : Anti-ALX3 antibody (ab64985) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : 293 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of HeLa cells, using ab64985 at 1/500-1/1000 dilution. Right hand panel was also treated with immunising peptide.

Anti-ALX3 antibody (ab64985)参考文献

ab64985 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue (Skin tissue)
Permeabilization No
Specification Skin tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
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提交于 May 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"