RabMAb

Anti-alpha Tubulin (phospho Y272)抗体[EP1334(2)Y] (ab76290)

概述

  • 产品名称
    Anti-alpha Tubulin (phospho Y272)抗体[EP1334(2)Y]
    参阅全部 alpha Tubulin 一抗
  • 描述
    兔单克隆抗体[EP1334(2)Y] to alpha Tubulin (phospho Y272)
  • 特异性
    This antibody detects alpha Tubulin phosphorylated at tyrosine 272.
  • 经测试应用
    适用于: WB, ICCmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho synthetic peptide corresponding to residues surrounding tyrosine 272 of human alpha Tubulin.

  • 阳性对照
    • Jurkat cell lysates
  • 常规说明

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab76290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/25000 - 1/50000. Predicted molecular weight: 54 kDa.
ICC 1/50 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能
      Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • 序列相似性
      Belongs to the tubulin family.
    • 翻译后修饰
      Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
      Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
    • 细胞定位
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha-tubulin 1 antibody
      • ALS22 antibody
      • B ALPHA 1 antibody
      • bA408E5.3 antibody
      • H2 ALPHA antibody
      • Hum a tub1 antibody
      • Hum a tub2 antibody
      • LIS3 antibody
      • MGC171407 antibody
      • MGC55332 antibody
      • TBA4A_HUMAN antibody
      • Testis-specific alpha-tubulin antibody
      • TUBA1 antibody
      • TUBA1A antibody
      • tuba1l antibody
      • Tuba4a antibody
      • Tubulin alpha 1 chain antibody
      • Tubulin alpha antibody
      • Tubulin alpha-1 chain antibody
      • tubulin alpha-1B chain antibody
      • Tubulin alpha-4A chain antibody
      • Tubulin H2-alpha antibody
      • Tubulin, alpha 1 (testis specific) antibody
      • tubulin, alpha 1, like antibody
      • Tubulin, alpha 4a antibody
      • Tubulin, alpha, testis-specific antibody
      • Tubulin, alpha-1 antibody
      see all

    图片

    • All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution

      Lane 1 : Jurkat cell lysates, untreated.
      Lane 2 : Jurkat cell lysates treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Paech F  et al. Hepatocellular Toxicity Associated with Tyrosine Kinase Inhibitors: Mitochondrial Damage and Inhibition of Glycolysis. Front Pharmacol 8:367 (2017). WB ; Human . Read more (PubMed: 28659801) »
    • Meshki J  et al. Substance P induces rapid and transient membrane blebbing in U373MG cells in a p21-activated kinase-dependent manner. PLoS One 6:e25332 (2011). WB . Read more (PubMed: 21966499) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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