Anti-alpha Tubulin抗体[YOL 1/34] (ab64332)

概述

  • 产品名称
    Anti-alpha Tubulin抗体[YOL 1/34]
    参阅全部 alpha Tubulin 一抗
  • 描述
    大鼠单克隆抗体[YOL 1/34] to alpha Tubulin
  • 特异性
    The antibody has a greater affinity for formaldehyde or glutaraldehyde fixed microtubules than native. The clone number has been updated from (2Q467) to (YOL 1/34) both clone numbers name the same antibody clone.
  • 经测试应用
    适用于: WB, ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: a wide range of other species, Mammal
  • 免疫原

    Full length protein (purified) (Human)

  • 表位
    ab64332 recognizes amino acids 414-422 of alpha Tubulin.

性能

应用

Our Abpromise guarantee covers the use of ab64332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 54 kDa.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent dilution.

靶标

  • 功能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 序列相似性
    Belongs to the tubulin family.
  • 翻译后修饰
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

图片

  • IHC image of alpha tubulin staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab64332, 5µg/ml overnight at +4°C. An anti-rat HRP (ab6734) secondary antibody (1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

文献

This product has been referenced in:
  • Nagaki K  et al. Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat. Front Plant Sci 6:912 (2015). IHC . Read more (PubMed: 26583020) »
  • Glass BJ  et al. The action of mimetic peptides on connexins protects fibroblasts from the negative effects of ischemia reperfusion. Biol Open 4:1473-80 (2015). WB . Read more (PubMed: 26471768) »

See all 3 Publications for this product

客户评价及客户问答

Application
ELISA
Sample
Human Serum (blood)
Specification
blood
Type
Sandwich (Capture)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Izabella Damm

Verified customer

提交于 Sep 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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