生物素Anti-alpha Tubulin抗体[TU-01] (ab74696)
Key features and details
- Biotin Mouse monoclonal [TU-01] to alpha Tubulin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
生物素Anti-alpha Tubulin抗体[TU-01]
参阅全部 alpha Tubulin 一抗 -
描述
生物素小鼠单克隆抗体[TU-01] to alpha Tubulin -
宿主
Mouse -
偶联物
Biotin -
特异性
ab74696 recognizes the defined epitope (aa 65-97) on the N-terminal structural domain of alpha-tubulin. This epitope is conserved within all species. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: a wide range of other species -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat, HeLa, HEK293T, U87-MG all under reducing conditions.
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常规说明
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Size exclusion -
克隆
单克隆 -
克隆编号
TU-01 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74696于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration. PubMed: 25665878
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WB |
Use a concentration of 1 - 2 µg/ml.
reducing conditions. |
说明 |
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IHC-P
Use at an assay dependent concentration. PubMed: 25665878 |
WB
Use a concentration of 1 - 2 µg/ml. reducing conditions. |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 7277 Human
- Omim: 191110 Human
- SwissProt: P68366 Human
- Unigene: 75318 Human
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别名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
图片
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Western blotting analysis labeling alpha-tubulin using ab74696 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of ab74696 followed by IRDye800-conjugated streptavidin. A specific band was detected for alpha-tubulin at approximately 54 kDa.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab74696 被引用在 3 文献中.
- Zeng Z et al. Joint tagging assisted fluctuation nanoscopy enables fast high-density super-resolution imaging. J Biophotonics 11:e201800020 (2018). PubMed: 29655260
- Chen X et al. Superior performance with sCMOS over EMCCD in super-resolution optical fluctuation imaging. J Biomed Opt 21:66007 (2016). PubMed: 27281064
- Zeng Z et al. Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging. Sci Rep 5:8359 (2015). IHC-P . PubMed: 25665878