Anti-alpha Tubulin抗体[TU-01] (ab7750)


  • 产品名称
    Anti-alpha Tubulin抗体[TU-01]
    参阅全部 alpha Tubulin 一抗
  • 描述
    小鼠单克隆抗体[TU-01] to alpha Tubulin
  • 经测试应用
    适用于: Flow Cyt, IHC-P, ELISA, ICC, ICC/IF, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Cow, Dog, Human, Pig
    预测可用于: Rat, Chicken, Xenopus laevis, Plants, a wide range of other species, Mammal, Xenopus tropicalis
  • 免疫原

    Full length native protein (purified) corresponding to Pig alpha Tubulin aa 37-154.

  • 表位
    aa 65-97 on N-terminal structural domain
  • 阳性对照
    • This antibody gave a positive signal in IF/ICC in HeLa cell line. Western Blot Positive control Pig Brain Lysate
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab216650 as a replacement.



Our Abpromise guarantee covers the use of ab7750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
ICC 1/100. For ICC, we recommend not only fixing but also permeabilizing the cells.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa.


  • 功能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 序列相似性
    Belongs to the tubulin family.
  • 翻译后修饰
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all


  • All lanes : Anti-alpha Tubulin antibody [TU-01] (ab7750) at 1/500 dilution

    Lane 1 : Dog 1 heart whole tissue lysate (left ventricle)
    Lane 2 : Dog 2 heart whole tissue lysate(left ventricle)
    Lane 3 : Dog 3 heart whole tissue lysate (left ventricle)
    Lane 4 : Dog 4 heart whole tissue lysate (left ventricle)

    Lysates/proteins at 80 µg per lane.

    HRP conjugated sheep polyclonal antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Exposure time : 20 minutes

    This image is courtesy of an Abreview submitted by Dr sudhish mishra

    See Abreview

  • ICC/IF image of ab7750 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7750, 5µg/ml) overnight at +4°C. The secondary antibody (green) was anti-mouse DyLight® 488 (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab7750 staining human normal skin. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with a retrieval buffer EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with an amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • Overlay histogram showing HEK293 cells stained with ab7750 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7750, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was anti-mouse DyLight® 488 (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence analysis of NIH 3T3 (mouse embryonal fibroblast) cells labelling alpha tubulin (green) with ab7750. Vimentin was stained red as counterstain. DAPI was used to stain the nuclei blue. 


This product has been referenced in:
  • Emerit MB  et al. A New Mechanism of Receptor Targeting by Interaction between Two Classes of Ligand-Gated Ion Channels. J Neurosci 36:1456-70 (2016). Read more (PubMed: 26843630) »
  • Sturm E  et al. Neuroprotection by Epigenetic Modulation in a Transgenic Model of Multiple System Atrophy. Neurotherapeutics 13:871-879 (2016). Read more (PubMed: 27259295) »

See all 16 Publications for this product


Western blot
Plants Tissue lysate - whole (Nicotiana benthamiana)
Gel Running Conditions
Non-reduced Denaturing (4-20% precut gels)
Loading amount
10 µg
lysis buffer 12h
Nicotiana benthamiana
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 20°C

Huan Zhang

Verified customer

提交于 Nov 13 2017

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Plants Cell (meiocyte)
Yes - Triton X-100

Abcam user community

Verified customer

提交于 Jun 23 2014

Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Human Cell lysate - other (U2OS)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Mar 17 2014

Thank you for contacting us.

The following antibody should be working with plant samples;

I hope this information is helpful to you. Please do not hesitate to cont...

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Thank you for contacting us and reporting the problems you have encountered in using the anti-alpha Tubulin antibody [TU-01] (ab7750).

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell lysate - whole cell (HEK 293T)
Total protein in input
350 µg
HEK 293T
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

提交于 Jan 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Hek 293)
Loading amount
10 µg
Hek 293
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C

Dr. Chris Tracy

Verified customer

提交于 Jan 12 2012

Thank you for your enquiry and your interest in our products. Anti-alpha tubulin (clone TU-01) can be used as loading control together with antibody HLA Class I (ab23755) . Currently, we have several forms of this antibody ie. unconjugated as well as c...

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Thank you for your enquiry. I can confirm that the loading control protein alpha tubulin should only be located in the cytoplasm. We have several antibodies for detection of alpha tubulin which have been tested in WB, human and pig. I can recommend ...

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I am sorry for the delayed response. We have not tested it in Fungi but we would expect it to react with Fungi. The secondary goat anti-mouse is compatible with ab7750. I hope this helps. Please do not hesitate to contact us if you have any further ...

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1-10 of 18 Abreviews or Q&A