HRP Anti-alpha Tubulin抗体[DM1A] - Loading Control (ab40742)
Key features and details
- HRP Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Xenopus laevis
- Conjugation: HRP
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
HRP Anti-alpha Tubulin抗体[DM1A] - Loading Control
参阅全部 alpha Tubulin 一抗 -
描述
HRP小鼠单克隆抗体[DM1A] to alpha Tubulin - Loading Control -
宿主
Mouse -
偶联物
HRP -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human, Xenopus laevis
预测可用于: Guinea pig -
免疫原
Full length native protein (purified) corresponding to Chicken alpha Tubulin.
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阳性对照
- This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Brain (Rat) Tissue Lysate - normal tissue Xenopus (Blastula) Whole Cell Lysate. IHC-P: FFPE human colon (normal) tissue sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
IgG fraction -
克隆
单克隆 -
克隆编号
DM1A -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184093)
- Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
- Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free (ab264493)
- FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
- Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
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Related Products
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- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- alpha Tubulin peptide (ab195584)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40742于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (5) |
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 50 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 50 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 7277 Human
- Entrez Gene: 22145 Mouse
- Entrez Gene: 316531 Rat
- Omim: 191110 Human
- SwissProt: P68366 Human
- SwissProt: P68368 Mouse
- SwissProt: Q5XIF6 Rat
- Unigene: 75318 Human
see all -
别名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
图片
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All lanes : HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :NIH/3T3 whole cell lysate (ab7179)
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Xenopus Laevis Protein Extracts (Stg 8)
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
IHC image of alpha Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab40742, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
数据表及文件
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SDS download
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Datasheet download
文献 (63)
ab40742 被引用在 63 文献中.
- Bakhshalizadeh S et al. Deficiency of the mitochondrial ribosomal subunit, MRPL50, causes autosomal recessive syndromic premature ovarian insufficiency. Hum Genet N/A:N/A (2023). WB . PubMed: 37148394
- Szaroszyk M et al. Skeletal muscle derived Musclin protects the heart during pathological overload. Nat Commun 13:149 (2022). PubMed: 35013221
- Siqueira E et al. Analysis of the circRNA and T-UCR populations identifies convergent pathways in mouse and human models of Rett syndrome. Mol Ther Nucleic Acids 27:621-644 (2022). PubMed: 35036070
- Shkarina K et al. Optogenetic activators of apoptosis, necroptosis, and pyroptosis. J Cell Biol 221:N/A (2022). PubMed: 35420640
- Artan M et al. Depletion of endogenously biotinylated carboxylases enhances the sensitivity of TurboID-mediated proximity labeling in Caenorhabditis elegans. J Biol Chem 298:102343 (2022). PubMed: 35933017