Anti-alpha Tubulin抗体[DM1A] - Loading Control (HRP) (ab40742)

概述

性能

应用

Our Abpromise guarantee covers the use of ab40742 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性
    Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase adds a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle. Alpha tubulin is not suitable as a loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1): 53-60, "in cells undergoing adipose differentiation actin synthesis decreases by 90%").
  • 细胞定位
    Major constituent of Microtubules
  • 数据库链接
  • 别名
    • TUBA1 antibody
    • TUBA1A antibody
    • TUBA2 antibody
    • TUBA3 antibody
    • TUBA3C antibody
    • TUBA4A antibody
    • Tubulin, alpha 1a antibody
    • Tubulin, alpha 3c antibody
    • Tubulin, alpha 4a antibody
    see all

Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP) 图像

  • IHC image of alpha Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab40742, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP) (ab40742) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 whole cell lysate (ab7179)
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Xenopus Laevis Protein Extracts (Stg 8)

    Lysates/proteins at 10 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

Anti-alpha Tubulin antibody [DM1A] - Loading Control (HRP) (ab40742)参考文献

This product has been referenced in:
  • de la Mare JA  et al. Extracellular Hsp90 and TGFß regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model. BMC Cancer 17:202 (2017). Human . Read more (PubMed: 28302086) »
  • Diaz-Lagares A  et al. Epigenetic inactivation of the p53-induced long noncoding RNA TP53 target 1 in human cancer. Proc Natl Acad Sci U S A 113:E7535-E7544 (2016). WB ; Human . Read more (PubMed: 27821766) »

See all 14 Publications for this product

Product Wall

Application
Western blot
Sample
Baboon Tissue lysate - whole (Fetal Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
12.5 µg
Specification
Fetal Skeletal muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Soon-Ok Kim

Verified customer

提交于 Feb 09 2017

Application
Western blot
Sample
Chicken Tissue lysate - whole (Chicken Embryo)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
Chicken Embryo
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Yuval Cinnamon

Verified customer

提交于 Dec 12 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Oct 12 2015

Thank you and your customer for taking the time to contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody after freezing.
The details your customer has kindly provided will provide us with...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Neonatal Rat cardiomyocytes)
Loading amount
20 µg
Specification
Neonatal Rat cardiomyocytes
Gel Running Conditions
Reduced Non-Denaturing (Native) (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Loading amount
50 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (8% Tris/HCL)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Feb 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册