Anti-alpha Synuclein (phospho S129)抗体(ab59264)

概述

  • 产品名称Anti-alpha Synuclein (phospho S129)抗体
    参阅全部 alpha Synuclein 一抗
  • 描述
    兔多克隆抗体to alpha Synuclein (phospho S129)
  • 特异性Detects endogenous levels of Synuclein only when phosphorylated at serine 129.
  • 经测试应用适用于: ICC/IF, IHC-P, ELISA, IHC-FoFr, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from Human Synuclein around the phosphorylation site of serine 129 (M-P-SP-E-E).

  • 阳性对照
    • Human brain.

性能

应用

Our Abpromise guarantee covers the use of ab59264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 22513881
IHC-P 1/50 - 1/100.
ELISA 1/5000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20098733
WB 1/500 - 1/1000.
IHC-Fr Use at an assay dependent concentration. PubMed: 23664753

靶标

  • 功能May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
  • 组织特异性Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
  • 疾病相关Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
    Parkinson disease 1
    Parkinson disease 4
    Dementia Lewy body
  • 序列相似性Belongs to the synuclein family.
  • 结构域The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
  • 翻译后修饰Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
    Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
    Ubiquitinated. The predominant conjugate is the diubiquitinated form.
    Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
  • 细胞定位Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha synuclein antibody
    • Alpha-synuclein antibody
    • Alpha-synuclein, isoform NACP140 antibody
    • alphaSYN antibody
    • MGC105443 antibody
    • MGC110988 antibody
    • MGC127560 antibody
    • MGC64356 antibody
    • NACP antibody
    • Non A beta component of AD amyloid antibody
    • Non A4 component of amyloid antibody
    • Non A4 component of amyloid precursor antibody
    • Non-A beta component of AD amyloid antibody
    • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
    • Non-A4 component of amyloid precursor antibody
    • Non-A4 component of amyloid, precursor of antibody
    • OTTHUMP00000218549 antibody
    • OTTHUMP00000218551 antibody
    • OTTHUMP00000218552 antibody
    • OTTHUMP00000218553 antibody
    • OTTHUMP00000218554 antibody
    • PARK 1 antibody
    • PARK 4 antibody
    • PARK1 antibody
    • PARK4 antibody
    • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
    • Parkinson disease familial 1 antibody
    • PD 1 antibody
    • PD1 antibody
    • SNCA antibody
    • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
    • SYN antibody
    • Synuclein alpha antibody
    • Synuclein alpha 140 antibody
    • Synuclein, alpha (non A4 component of amyloid precursor) antibody
    • SYUA_HUMAN antibody
    see all

Anti-alpha Synuclein (phospho S129) antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59264 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide.
  • All lanes : Anti-alpha Synuclein (phospho S129) antibody (ab59264) at 1/500 dilution

    Lane 1 : Mouse brain whole cell lysates
    Lane 2 : Mouse brain whole cell lysates with immunogen phosphopeptide
  • All lanes : Anti-alpha Synuclein (phospho S129) antibody (ab59264) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from young transgenic mouse overexpressing human alpha-synuclein
    Lane 2 : Whole tissue lysate prepared from young transgenic mouse overexpressing human alpha-synuclein
    Lane 3 : Whole tissue lysate prepared from young transgenic mouse overexpressing human alpha-synuclein
    Lane 4 : Whole tissue lysate prepared from old transgenic mouse overexpressing human alpha-synuclein
    Lane 5 : Whole tissue lysate prepared from old transgenic mouse overexpressing human alpha-synuclein
    Lane 6 : Whole tissue lysate prepared from old transgenic mouse overexpressing human alpha-synuclein
    Lane 7 : Whole tissue lysate prepared from KO mouse

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat anti-rabbit Ig (H+L) HRP at 1/1000 dilution
    Developed using the ECL technique

    Observed band size : 18,19 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    Image courtesy of Sébastien Brot by Abreview.

    See Abreview

Anti-alpha Synuclein (phospho S129) antibody (ab59264)参考文献

This product has been referenced in:
  • Aulic S  et al. Defined a-synuclein prion-like molecular assemblies spreading in cell culture. BMC Neurosci 15:69 (2014). ICC/IF ; Human . Read more (PubMed: 24898419) »
  • Aldrin-Kirk P  et al. Novel AAV-based rat model of forebrain synucleinopathy shows extensive pathologies and progressive loss of cholinergic interneurons. PLoS One 9:e100869 (2014). IHC-Fr ; Mouse . Read more (PubMed: 24999658) »

See all 12 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (MCF7 (Human breast adenocarcinoma cell line))
Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount 20 µg
Specification MCF7 (Human breast adenocarcinoma cell line)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Julien Chevalier

Verified customer

提交于 Jul 20 2016

Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 50 µg
Treatment alpha synuclein
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 23 2015

Thank you for contacting us.

The price for antibody as per your requirement is Euro 370 plus Euro 42 delivery charges. Please check the datasheet; http://www.abcam.com/alpha-Synuclein-phospho-S129-antibody-ab59264.html

I hope this inf...

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Thank you for contacting Abcam.
I have been in contact with the lab and they have told me that for ab59264, the following reactions were showed positive:
1) Peptide-Coated ELISA: Coat immunogen phospho-peptide and non-phospho-peptide (pair) i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (gel 15 %)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Sébastien Brot

Verified customer

提交于 Jul 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"