Anti-Alpha-synuclein (phospho S129)抗体(ab59264)
Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho S129)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Alpha-synuclein (phospho S129)抗体
参阅全部 Alpha-synuclein 一抗 -
描述
兔多克隆抗体to Alpha-synuclein (phospho S129) -
宿主
Rabbit -
特异性
Detects endogenous levels of Synuclein only when phosphorylated at serine 129. Due to 69% sequence homology ab59264 might react with Beta synuclein.
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经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Alpha-synuclein (phospho S129). Synthetic phosphopeptide derived from Human Synuclein around the phosphorylation site of serine 129 (M-P-SP-E-E).
Database link: P37840 -
阳性对照
- Human and mouse brain.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59264于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50 - 1/100.
Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes. Primary antibody incubation: 1 hour at 37°C Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 µL for 30 minutes. DAB reagent. |
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WB | (3) |
1/500 - 1/1000.
Please see WB protocol details in the image legend. |
说明 |
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IHC-P
1/50 - 1/100. Antigen retrieval: Microwave method - put the slice into 10 mmol/L citrate buffer (pH 6.0), microwave high temperature for 5 minutes, and then medium temperature for 15 minutes. Primary antibody incubation: 1 hour at 37°C Secondary antibody: Poly-HRP-Anti Mouse/Rabbit IgG, 50 µL for 30 minutes. DAB reagent. |
WB
1/500 - 1/1000. Please see WB protocol details in the image legend. |
靶标
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功能
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
组织特异性
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
疾病相关
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
序列相似性
Belongs to the synuclein family. -
结构域
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
翻译后修饰
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
细胞定位
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 6622 Human
- Entrez Gene: 20617 Mouse
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: O55042 Mouse
- Unigene: 21374 Human
- Unigene: 17484 Mouse
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别名
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
图片
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All lanes : Anti-Alpha-synuclein (phospho S129) antibody (ab59264) at 1/500 dilution
Lane 1 : Mouse brain whole cell lysates
Lane 2 : Mouse brain whole cell lysates with immunogen phosphopeptide
Lysates/proteins at 40 µg per lane.Blocking buffer: 5% (w/v) BSA in TBST.
Primary antibody dilution buffer: 5%(w/v)BSA,0.1%(v/v), Tween-20 in TBST.
Secondary antibody dilution buffer: 5%(w/v)BSA,0.1%(v/v),Tween-20 in TBST.
12% SDS gel. Nitrocellulose membrane.
Blocking: Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.
Primary antibody incubation: diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Secondary antibody incubation: diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
ECL development.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59264 at a dilution of 1/50-1/100. Left hand image - without immunising peptide; right hand image - with immunising peptide.
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All lanes : Anti-Alpha-synuclein (phospho S129) antibody (ab59264) at 1/1000 dilution
Lanes 1-3 : Whole tissue lysate prepared from young transgenic mouse overexpressing human alpha-synuclein
Lanes 4-6 : Whole tissue lysate prepared from old transgenic mouse overexpressing human alpha-synuclein
Lane 7 : Whole tissue lysate prepared from KO mouse
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-rabbit Ig (H+L) HRP at 1/1000 dilution
Developed using the ECL technique.
Observed band size: 18,19 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (57)
ab59264 被引用在 57 文献中.
- Li ZF et al. A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease. Dis Model Mech 15:N/A (2022). PubMed: 35023548
- Choi M et al. Conformation-specific Antibodies Targeting Aggregated Forms of α-synuclein Block the Propagation of Synucleinopathy. Exp Neurobiol 31:29-41 (2022). PubMed: 35256542
- Santulli C et al. Neuronal haemoglobin induces loss of dopaminergic neurons in mouse Substantia nigra, cognitive deficits and cleavage of endogenous α-synuclein. Cell Death Dis 13:1048 (2022). PubMed: 36526614
- Forrest SL et al. Coexisting Lewy body disease and clinical parkinsonism in amyotrophic lateral sclerosis. Eur J Neurol 28:2192-2199 (2021). PubMed: 33793036
- Moors TE et al. The subcellular arrangement of alpha-synuclein proteoforms in the Parkinson's disease brain as revealed by multicolor STED microscopy. Acta Neuropathol 142:423-448 (2021). PubMed: 34115198