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Lewy bodies purified from patients suffering dementia with Lewy bodies
Our Abpromise guarantee covers the use of ab27766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||1/100 - 1/10000. Predicted molecular weight: 14 kDa.|
|IHC-Fr||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000. Do not perform antigen retrieval.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC||Use at an assay dependent concentration.|
ab27766 staining alpha Synuclein in Rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 5% BSA for 1 hour at 37°C. Samples were incubated with primary antibody (1/100) for 18 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"