Anti-alpha smooth muscle Actin抗体, prediluted (ab15267)

概述

  • 产品名称Anti-alpha smooth muscle Actin抗体, prediluted
    参阅全部 alpha smooth muscle Actin 一抗
  • 描述
    兔多克隆抗体to alpha smooth muscle Actin, prediluted
  • 经测试应用适用于: IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cat, Human
    预测可用于: Rabbit, Chicken, Cow, Baboon
  • 免疫原

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal).

  • 阳性对照
    • Smooth muscle or leiomyosarcoma.

性能

应用

Our Abpromise guarantee covers the use of ab15267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
IHC-P
  • 应用说明IHC-P: Ready to use for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
    IHC-Fr: Use at an assay dependent dilution (from PubMed:17149535).
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    Anti-alpha smooth muscle Actin antibody, prediluted 图像

    • ab15267 staining alpha smooth muscle Actin in human leiomyoma by Immunohistochemistry (FFPE-sections).

    • ab15267 (used X1) staining day 3 wounded rat skin tissue by IHC-P. The tissue was formaldehyde fixed and blocked with peroxidase prior to incubation with the antibody for 25 minutes. An HRP conuugated goat polyclonal antibody was used as the secondary.

      See Abreview

    Anti-alpha smooth muscle Actin antibody, prediluted (ab15267)参考文献

    This product has been referenced in:
    • Evans DJ  et al. Platelet P2Y(12) receptor influences the vessel wall response to arterial injury and thrombosis. Circulation 119:116-22 (2009). IHC-P ; Mouse . Read more (PubMed: 19103996) »
    • Maclauchlan S  et al. Enhanced angiogenesis and reduced contraction in thrombospondin-2-null wounds is associated with increased levels of matrix metalloproteinases-2 and -9, and soluble VEGF. J Histochem Cytochem 57:301-13 (2009). Read more (PubMed: 19029404) »

    See all 7 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step solution from Dako as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
    Antigen retrieval step None
    Sample Mouse Tissue sections (kidney)
    Specification kidney
    Permeabilization No
    Fixative Formaldehyde
    Username

    Dr. R Berahovich

    Verified customer

    提交于 Aug 06 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Cat Tissue sections (Skin)
    Specification Skin
    Fixative Formaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Abcam user community

    Verified customer

    提交于 Jan 04 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Wound)
    Specification Wound
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 09 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Skin)
    Specification Skin
    Fixative Formaldehyde
    Antigen retrieval step None
    Blocking step peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 5
    Username

    Dr. Philip Roby

    Verified customer

    提交于 Nov 23 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"