Anti-alpha smooth muscle Actin抗体[4A4] (ab119952)


  • 产品名称Anti-alpha smooth muscle Actin抗体[4A4]
    参阅全部 alpha smooth muscle Actin 一抗
  • 描述
    小鼠单克隆抗体[4A4] to alpha smooth muscle Actin
  • 经测试应用适用于: WB, ELISA, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Monkey
  • 免疫原

    Synthetic peptide of Human alpha smooth muscle Actin (C-EYDEAGPSIVHRKCF) conjugated KLH

  • 阳性对照
    • Recombinant alpha smooth muscle Actin protein, Hela, A431, Jurkat, K562, HEK293, HepG2, NIH3T3, PC12 and COS7 cell lysates, HepG2 cells, HeLa cells



Our Abpromise guarantee covers the use of ab119952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.


  • 功能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 疾病相关Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
  • 序列相似性Belongs to the actin family.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • a actin antibody
    • AAT6 antibody
    • ACTA_HUMAN antibody
    • ACTA2 antibody
    • Actin alpha 2 smooth muscle aorta antibody
    • Actin aortic smooth muscle antibody
    • Actin, aortic smooth muscle antibody
    • ACTSA antibody
    • ACTVS antibody
    • Alpha 2 actin antibody
    • Alpha actin 2 antibody
    • Alpha cardiac actin antibody
    • Alpha-actin-2 antibody
    • Cell growth inhibiting gene 46 protein antibody
    • Cell growth-inhibiting gene 46 protein antibody
    • GIG46 antibody
    • Growth inhibiting gene 46 antibody
    • MYMY5 antibody
    see all

Anti-alpha smooth muscle Actin antibody [4A4] 图像

  • All lanes : Anti-alpha smooth muscle Actin antibody [4A4] (ab119952) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : HEK293 cell lysate
    Lane 6 : HepG2 cell lysate
    Lane 7 : NIH3T3 cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : COS7 cell lysate

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • Immunohistochemical analysis of paraffin-embedded Human duodenum tissue using ab119952 at a dilution of 1/200 with DAB staining.
  • Immunohistochemical analysis of paraffin-embedded Human esophagus tissue using ab119952 at a dilution of 1/200 with DAB staining.
  • Immunofluorescence analysis of HepG2 cells using ab119952 at 1/200 (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (Red). DRAQ5 fluorescent DNA dye (blue).
  • Flow cytometric analysis of Hela cells using ab119952 at a dilution of 1/200 (green) and negative control (purple).
  • ELISA, using ab119952 at a dilution of 1/10000.

Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)参考文献

ab119952 has not yet been referenced specifically in any publications.

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I can confirm that these three antibodies recognize the mouse protein and the host species in which they were raised is also mouse. This may cause some non-specificity signal problem mainly in immunohistochemical using tissue sections (mouse-on-mouse) ...

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The histogram showed in the datasheet was obtained using 4% paraformaldehyde as fixative agent, and 0.1% Triton-X100 and 0.5% BSA in 1X PBS to permeabilise.