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The N-terminal decapeptide of alpha-smooth muscle actin.
This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary. This antibody strongly crossreacts with alphasmooth muscle actin in a variety of mammalian and non-mammalian species.
The antibody is conjugated with FITC at a ratio 6 Moles of Fluorescein to 1 Mole of antibody. Maximum excitation of FITC occurs at wavelength 492 nm, and maximum emission occurs at wavelenght 520 nm.
Our Abpromise guarantee covers the use of ab8211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 17222057
ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/75. (as well as on cell smears).|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. PubMed: 17237513Use at an assay dependent dilution (from Pubmed:17237513).|
ab8211 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8211 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 4% formaldehyde (10 min).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"