Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung, smooth muscle cell)
Permeabilization
Yes - sodium citrate buffer
Specification
lung, smooth muscle cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: donkey anti-goat-alexa 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Good results wasn't obtained by the staining procedure without permeabilization. Permeabilization is required.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 14 2016