Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Treatment
Day 0 and day 7 post-MI
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase goat anti-rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Pierce ECL
Bands
Specific: 42 kDa
Exposure
2 second(s)
Additional data
Additional Notes
Immunoblotting of day 0 left ventricle control or day 7 post MI infarct region in C57/BL6J wild type mice. Primary dilution 1:1000 in 5% milk,
secondary dilution 1:5000 in 5% milk.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Merry Lindsey
Verified customer
提交于 Nov 28 2013