Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel)
Loading amount
10 µg
Treatment
day 0 and day 7 after myocardial infarction
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Peroxidase goat anti-rabbit IgG secondary antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Pierce ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 42 kDa Non-specific: 50, 87 kDa
Additional data
Additional Notes
Primary dilution 1:1000 in 5% milk
Secondary dilution 1:5000 in 5% milk
Very faint bands also seen at 50 and 87 kDa only in the day 0 samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Merry Lindsey
Verified customer
提交于 Sep 20 2013