Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Aortic smooth muscle cells)
Specification
Aortic smooth muscle cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-X/methanol
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Other product details
Dilution
1/25
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Definitely not useful for immunocytochemistry - tried both cytospins and cultured cells from aorta. No proper signal.
Abcam response
Thank you for your honest feedback about this antibody. As this antibody had not previously been tested in mouse or in ICC/IF, it was previously unknown whether this antibody would be suitable for use in this species and application. As the antibody has been used in flow cytometry, it seems likely to also work in ICC, so we suspect that the antibody may simply not recognize the protein in mouse.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 29 2008