存放说明Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
溶解度概述Soluble in water
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
ab24851 staining cAMP in MALME-3M cells treated with α-MSH (ab120205), by ICC/IF. Increase of cAMP correlates with increased concentration of α-MSH, as described in literature. The cells were incubated at 37°C for 2h in media containing different concentrations of ab120205 (α-MSH) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab24851 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
α-MSH (free acid) (ab120205)参考文献
This product has been referenced in:
Luger TA & Brzoska T alpha-MSH related peptides: a new class of anti-inflammatory and immunomodulating drugs. Ann Rheum Dis66 Suppl 3:iii52-5 (2007).
Read more (PubMed: 17934097) »
Wu CS et al. Peripherally administered desacetyl alpha-MSH and alpha-MSH both influence postnatal rat growth and associated rat hypothalamic protein expression. Am J Physiol Endocrinol Metab291:E1372-80 (2006).
Read more (PubMed: 16868223) »