Anti-alpha Lactalbumin抗体[F20.16] (ab20212)

概述

  • 产品名称Anti-alpha Lactalbumin抗体[F20.16]
    参阅全部 alpha Lactalbumin 一抗
  • 描述
    小鼠单克隆抗体[F20.16] to alpha Lactalbumin
  • 经测试应用适用于: WB, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified Alpha Lactalbumin

  • 常规说明


    This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Purified IgM
  • 纯化说明This antibody was purified using affinity chromatography. The sample was eluted using a commercially bought Neutral Elution Buffer and exchanged into PBS using a G25 column.
  • Primary antibody说明This antibody can be used as an in vitro marker of terminally differentiated breast lumenal epithelial cells.
  • 克隆单克隆
  • 克隆编号F20.16
  • 骨髓瘤x63-Ag8.653
  • 同种型IgM
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab20212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 14 kDa.
IHC-Fr Use at an assay dependent concentration.

Use withn acetone fixed tissues.

ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.
  • 组织特异性Mammary gland specific. Secreted in milk.
  • 序列相似性Belongs to the glycosyl hydrolase 22 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • a-LACTA antibody
    • alfaLA antibody
    • Alpha-lactalbumin antibody
    • Lactalbumin alpha antibody
    • Lactose synthase B protein antibody
    • LALBA antibody
    • LALBA_HUMAN antibody
    • Lysozyme-like protein 7 antibody
    • LYZL7 antibody
    • MGC138521 antibody
    • MGC138523 antibody
    see all

Anti-alpha Lactalbumin antibody [F20.16] 图像

  • ICC/IF image of ab20212 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20212, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-alpha Lactalbumin antibody [F20.16] (ab20212)参考文献

This product has been referenced in:
  • Burchell J  et al. Production and characterization of monoclonal antibodies to human casein. A monoclonal antibody that cross-reacts with casein and alpha-lactalbumin. Hybridoma 4:341-50 (1985). Read more (PubMed: 3905581) »

See 1 Publication for this product

Product Wall

The histology was carried out on frozen sections (10ug) of lactating mammary glands.

It seems that lactating tissue is the most appropriate positive control, as normal and cancerous tissue does not appear to express alpha lactalbumin accordi...

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Thank you for contacting us. Unfortunately, the antibodies mentioned are not suitable to detect these (human) proteins in ELISA. However, we have the following antibodies available: 1) Beta-lactoglobulin: Unfortunately, I was unable to find a human ort...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"