RabMAb

Anti-alpha Internexin抗体[EP676Y] (ab40758)

概述

  • 产品名称Anti-alpha Internexin抗体[EP676Y]
    参阅全部 alpha Internexin 一抗
  • 描述
    兔单克隆抗体[EP676Y] to alpha Internexin
  • 经测试应用适用于: IHC-FoFr, WB, Flow Cyt, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    不与反应: Apteronotus leptorhynchus
  • 免疫原

    Synthetic peptide corresponding to residues in the tail domain (Human)

  • 阳性对照
    • SHSY5Y cell lysate, human brain
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab40758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
WB 1/5000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 66 kDa).
Flow Cyt 1/25.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
IHC-P 1/250 - 1/500.
ICC/IF 1/250 - 1/500.

靶标

  • 功能Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • 组织特异性Found predominantly in adult CNS.
  • 序列相似性Belongs to the intermediate filament family.
  • 发展阶段Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • 翻译后修饰O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

Anti-alpha Internexin antibody [EP676Y] 图像

  • Anti-alpha Internexin antibody [EP676Y] (ab40758) at 1/10000 dilution + SHSY5Y cell lysate at 10 µg

    Predicted band size : 66 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab40758 staining alpha Internexin in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, permeablized with 0.01% Triton X-100, blocked with 5% serum for 2 hours at 4°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/250) at 4°C for 10 hours. An undiluted Alexa Fluor® 555-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.

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  • Positive staining of alpha Internexin on paraffin-embedded human brain tissue (A) using ab40758 diluited 1:250. Negative staining of paraffin-embedded human colon (B), Kidney (C) and uterus (D) tissues using ab40758 under the same conditions.
  • ab40758 staining alpha Internexin in rat glial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 10 hours at 4°C. An undiluted Alexa Fluor® 594-conjugated donkey anti-rabbit IgG polyclonal was used as the secondary antibody.

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  • Immunofluorescent staining of alpha Internexin on PC 12 cells using ab40758 diluted 1:250.

Anti-alpha Internexin antibody [EP676Y] (ab40758)参考文献

This product has been referenced in:
  • Louis ED  et al. Neurofilament protein levels: quantitative analysis in essential tremor cerebellar cortex. Neurosci Lett 518:49-54 (2012). Read more (PubMed: 22561033) »

See 1 Publication for this product

Product Wall

Application IHC - Wholemount
Sample Human Tissue (Brain)
Specification Brain
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Abcam user community

Verified customer

提交于 Dec 29 2014

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (Mixed Glial cells)
Specification Mixed Glial cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 21 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.01% Triton X100
Fixative Formaldehyde
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Abcam user community

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提交于 Jul 17 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Rat Cell (Glial Cells)
Specification Glial Cells
Permeabilization Yes - Saponin
Fixative Paraformaldehyde
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Abcam user community

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提交于 Jun 13 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

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提交于 Feb 06 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 Apr 12 2012


I am very pleased to hear you would like to accept our offer and test the above antivbodies in Apteronotus leptorhynchus. This code will give you 1 free primary antibody before the expiration date for each code. To redeem this offer, please submit...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (brain)
Specification brain
Fixative Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Vlady Pavlova

Verified customer

提交于 Dec 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"