Anti-alpha Glucosidase II抗体(ab69489)


  • 产品名称Anti-alpha Glucosidase II抗体
    参阅全部 alpha Glucosidase II 一抗
  • 描述
    兔多克隆抗体to alpha Glucosidase II
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Hamster, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 916-930 of Human alpha Glucosidase II, prepared as a multiple antigen peptide (MAP).

  • 阳性对照
    • Rat Brain Tissue Extract This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: HeLa and HepG2.



Our Abpromise guarantee covers the use of ab69489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明IP: 1/50.
    WB: 1/500 sufficient for detection of a-Glucosidase II in 20µg of rat brain tissue extract by colorimetric analysis.
    It is recommended for Western blot applications, that the secondary antibody be quenched by adding normal sera (of an identical species to secondary host) to the diluent. Detects a band of approximately 110 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins.
    • 组织特异性Detected in placenta.
    • 通路Glycan metabolism; N-glycan metabolism.
    • 序列相似性Belongs to the glycosyl hydrolase 31 family.
    • 细胞定位Endoplasmic reticulum. Golgi apparatus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha glucosidase II alpha subunit antibody
      • Alpha-glucosidase 2 antibody
      • G2AN antibody
      • GANAB antibody
      • GANAB_HUMAN antibody
      • Glu II antibody
      • Glucosidase alpha neutral AB antibody
      • Glucosidase II alpha antibody
      • Glucosidase II subunit alpha antibody
      • GluII antibody
      • KIAA0088 antibody
      • Neutral alpha glucosidase AB antibody
      • Neutral alpha glucosidase AB precursor antibody
      • Neutral alpha-glucosidase AB antibody
      see all

    Anti-alpha Glucosidase II antibody 图像

    • ICC/IF image of ab69489 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab69489 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in methanol (100%, 5min) and formaldehyde (4%, 10min) fixed HepG2 cells at 1/200 dilution.

    Anti-alpha Glucosidase II antibody (ab69489)参考文献

    This product has been referenced in:
    • Iwamoto S  et al. Reconstructed glycan profile for evaluation of operating status of the endoplasmic reticulum glycoprotein quality control. Glycobiology 23:121-31 (2013). Read more (PubMed: 22975980) »
    • Dai L  et al. Differential profiling studies of N-linked glycoproteins in glioblastoma cancer stem cells upon treatment with ?-secretase inhibitor. Proteomics 11:4021-8 (2011). Read more (PubMed: 21898824) »

    See all 3 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Human Cell (LNCaP)
    Specification LNCaP
    Permeabilization No
    Fixative Paraformaldehyde

    Dr. Armen Petrosyan

    Verified customer

    提交于 Nov 14 2013