RabMAb

Anti-Alpha Fodrin抗体[EPR3830] (ab92373)

概述

  • 产品名称Anti-Alpha Fodrin抗体[EPR3830]
    参阅全部 Alpha Fodrin 一抗
  • 描述
    兔单克隆抗体[EPR3830] to Alpha Fodrin
  • 特异性Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185.
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Alpha Fodrin.

  • 阳性对照
    • HeLa + Camptothecin cell lysate, HeLa cell lysate, Jurkat + Staurosporine cell lysate, Jurkat cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab92373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明

    IP: 1/10 - 1/100.
    WB: 1/10,000 - 1/50,000. Predicted molecular weight: 285 kDa.

    Is unsuitable for Flow Cyt, ICC or IHC-P.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
    • 疾病相关Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
    • 序列相似性Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 23 spectrin repeats.
    • 翻译后修饰Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
    • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • (ALPHA)II-SPECTRIN antibody
      • Alpha II Spectrin antibody
      • Alpha-II spectrin antibody
      • brain antibody
      • EIEE5 antibody
      • FLJ17738 antibody
      • FLJ44613 antibody
      • Fodrin alpha chain antibody
      • Fodrin, alpha antibody
      • NEAS antibody
      • Non erythrocytic spectrin alpha antibody
      • non-erythroid alpha chain antibody
      • SPECA antibody
      • Spectrin alpha chain antibody
      • Spectrin alpha chain brain antibody
      • Spectrin alpha non erythrocytic 1 antibody
      • Spectrin antibody
      • Spectrin non erythroid alpha chain antibody
      • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
      • Spectrin, nonerythroid, alpha subunit antibody
      • Spna2 antibody
      • SPTA 2 antibody
      • SPTA2 antibody
      • SPTA2_HUMAN antibody
      • SPTAN 1 antibody
      • SPTAN1 antibody
      see all

    Anti-Alpha Fodrin antibody [EPR3830] 图像

    • All lanes : Anti-Alpha Fodrin antibody [EPR3830] (ab92373)

      Lane 1 : HeLa + Camptothecin cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat + Staurosporine cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 285 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Anti-Alpha Fodrin antibody [EPR3830] (ab92373)参考文献

    ab92373 has not yet been referenced specifically in any publications.

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