概述

  • 产品名称
    Anti-Alpha Fodrin抗体[D8B7]
    参阅全部 Alpha Fodrin 一抗
  • 描述
    小鼠单克隆抗体[D8B7] to Alpha Fodrin
  • 经测试应用
    适用于: ICC/IF, IP, IHC-P, ELISA, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Human, African green monkey, Chinese hamster
  • 免疫原

    Full length native protein (purified) corresponding to Human Alpha Fodrin.

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab133320 as a replacement.

性能

应用

Our Abpromise guarantee covers the use of ab11755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21059848
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. See Abreviews.
ELISA 1/100000 - 1/1e+006.
WB 1/100 - 1/1000. Predicted molecular weight: 297 kDa.
IHC-Fr 1/10 - 1/50.

靶标

  • 功能
    Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
  • 疾病相关
    Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
  • 序列相似性
    Belongs to the spectrin family.
    Contains 3 EF-hand domains.
    Contains 1 SH3 domain.
    Contains 23 spectrin repeats.
  • 翻译后修饰
    Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
  • 细胞定位
    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • (ALPHA)II-SPECTRIN antibody
    • Alpha II Spectrin antibody
    • Alpha-II spectrin antibody
    • brain antibody
    • EIEE5 antibody
    • FLJ17738 antibody
    • FLJ44613 antibody
    • Fodrin alpha chain antibody
    • Fodrin, alpha antibody
    • NEAS antibody
    • Non erythrocytic spectrin alpha antibody
    • non-erythroid alpha chain antibody
    • SPECA antibody
    • Spectrin alpha chain antibody
    • Spectrin alpha chain brain antibody
    • Spectrin alpha non erythrocytic 1 antibody
    • Spectrin antibody
    • Spectrin non erythroid alpha chain antibody
    • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
    • Spectrin, nonerythroid, alpha subunit antibody
    • Spna2 antibody
    • SPTA 2 antibody
    • SPTA2 antibody
    • SPTA2_HUMAN antibody
    • SPTAN 1 antibody
    • SPTAN1 antibody
    see all

图片

  • IF using ab11755.
  • ab11755 at 1/100 staining mouse gut (small bowel) tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was blocked and incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

    See Abreview

  • Anti-Alpha Fodrin antibody [D8B7] (ab11755) at 1/1000 dilution + 3T3 whole cell lysate at 30 µg

    Secondary
    HRP conjugated goat anti-mouse at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 297 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

文献

This product has been referenced in:
  • Yamamoto K  et al. The neuroprotective effect of latanoprost acts via klotho-mediated suppression of calpain activation after optic nerve transection. J Neurochem 140:495-508 (2017). WB . Read more (PubMed: 27859240) »
  • Liu M  et al. Heme-mediated apoptosis and fusion damage in BeWo trophoblast cells. Sci Rep 6:36193 (2016). Read more (PubMed: 27796349) »

See all 10 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing
Sample
Sheep Tissue lysate - other (cardiac)
Specification
cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa S3)
Specification
HeLa S3
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

提交于 Oct 30 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Cell lysate - whole cell (PC12)
Total protein in input
500 µg
Specification
PC12
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

提交于 Oct 30 2007

Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO cell line)
Loading amount
30 µg
Specification
CHO cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Sep 18 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
Loading amount
30 µg
Specification
Cos-7 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Sep 18 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 cell line)
Loading amount
30 µg
Specification
3T3 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Sep 18 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (anal cancer)
Specification
anal cancer
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 31 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (gut (small bowel))
Specification
gut (small bowel)
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 31 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12)
Loading amount
40 µg
Specification
PC12
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jul 11 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
40 µg
Specification
U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jul 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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