Anti-Alpha Fodrin抗体(ab74312)


  • 产品名称Anti-Alpha Fodrin抗体
    参阅全部 Alpha Fodrin 一抗
  • 描述
    兔多克隆抗体to Alpha Fodrin
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Medaka fish
  • 免疫原

    Synthetic peptide from a region between residues 2422 and 2472 of human Alpha Fodrin (NP_003118.1)

  • 阳性对照
    • Whole cell lysate from HeLa, 293T or NIH3T3 cells



Our Abpromise guarantee covers the use of ab74312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 285 kDa.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
    • 疾病相关Defects in SPTAN1 are the cause of epileptic encephalopathy early infantile type 5 (EIEE5) [MIM:613477]. EIEE5 is a disorder characterized by seizures associated with hypsarrhythmia profound mental retardation with lack of visual attention and speech development, as well as spastic quadriplegia.
    • 序列相似性Belongs to the spectrin family.
      Contains 3 EF-hand domains.
      Contains 1 SH3 domain.
      Contains 23 spectrin repeats.
    • 翻译后修饰Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro.
    • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Expressed along the cell membrane in podocytes and presumptive tubule cells during glomerulogenesis and is expressed along lateral cell margins in tubule cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • (ALPHA)II-SPECTRIN antibody
      • Alpha II Spectrin antibody
      • Alpha-II spectrin antibody
      • brain antibody
      • EIEE5 antibody
      • FLJ17738 antibody
      • FLJ44613 antibody
      • Fodrin alpha chain antibody
      • Fodrin, alpha antibody
      • NEAS antibody
      • Non erythrocytic spectrin alpha antibody
      • non-erythroid alpha chain antibody
      • SPECA antibody
      • Spectrin alpha chain antibody
      • Spectrin alpha chain brain antibody
      • Spectrin alpha non erythrocytic 1 antibody
      • Spectrin antibody
      • Spectrin non erythroid alpha chain antibody
      • Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) antibody
      • Spectrin, nonerythroid, alpha subunit antibody
      • Spna2 antibody
      • SPTA 2 antibody
      • SPTA2 antibody
      • SPTA2_HUMAN antibody
      • SPTAN 1 antibody
      • SPTAN1 antibody
      see all

    Anti-Alpha Fodrin antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Alpha Fodrin with ab74312 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-Alpha Fodrin antibody (ab74312) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

      developed using the ECL technique

      Predicted band size : 285 kDa
      Observed band size : 285 kDa

      Exposure time : 3 seconds

    Anti-Alpha Fodrin antibody (ab74312)参考文献

    ab74312 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab74312.
    Please use the links above to contact us or submit feedback about this product.