概述

  • 产品名称Anti-alpha COP I抗体
    参阅全部 alpha COP I 一抗
  • 描述
    兔多克隆抗体to alpha COP I
  • 特异性Detects coatomer-protein I alpha (COP I alpha).
  • 经测试应用适用于: IP, ICC, WBmore details
  • 种属反应性
    与反应: Rat, Human, Non Human Primates
    预测可用于: Mouse, Cow, Dog, Rice
  • 免疫原

    Synthetic peptide corresponding to Rat alpha COP I aa 1-19.
    Sequence:

    MLTKFETKSARVKGLSFHP


    (Peptide available as ab4931)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab2913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
ICC Use a concentration of 3 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100, 140 kDa.Can be blocked with alpha COP I peptide (ab4931).

靶标

  • 功能The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
    Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.
  • 组织特异性Uniformly expressed in a wide range of adult and fetal tissues. Xenin is found in gastric, duodenal and jejunal mucosa. Circulates in the blood. Seems to be confined to specific endocrine cells.
  • 序列相似性Contains 6 WD repeats.
  • 发展阶段Xenin is released into the circulation after a meal.
  • 细胞定位Secreted and Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha coat protein antibody
    • Alpha COP antibody
    • Alpha COPI antibody
    • Alpha-coat protein antibody
    • Alpha-COP antibody
    • AlphaCOP antibody
    • Coatomer protein complex subunit alpha antibody
    • Coatomer subunit alpha antibody
    • COP A antibody
    • COP I alpha antibody
    • copA antibody
    • COPA_HUMAN antibody
    • COPI alpha antibody
    • FLJ26320 antibody
    • HEP COP antibody
    • HEP-COP antibody
    • HEPCOP antibody
    • Proxenin antibody
    • Xenin antibody
    • Xenopsin-related peptide antibody
    see all

Anti-alpha COP I antibody 图像

  • ICC/IF of COP1 in Hela Cells

Anti-alpha COP I antibody (ab2913)参考文献

This product has been referenced in:
  • Sudo H  et al. Knockdown of COPA, identified by loss-of-function screen, induces apoptosis and suppresses tumor growth in mesothelioma mouse model. Genomics 95:210-6 (2010). WB ; Human . Read more (PubMed: 20153416) »

See 1 Publication for this product

Product Wall

Thank you for the explanation, and for the image and protocol. The staining you are seeing with the alpha COP anditboy ab2913 does look incorrect, if all the staining is expected to be peri-nuclear, as is seen with the delta COP antibody. The small ...

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Thank you for contacting us. I am sorry to read that you are having trouble getting a good result with this antibody. Can you please tell me what application you are using it for? I am assuming IHC or ICC but I will need a few more details. We alway...

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Thank you for your email. Ab2913 was tested for application in Western blotting using PC-12 cell extract. The antibody detected a band at approximately 140 kDa representing COPI alpha. The antibody also detected a band at approximately 100 kDa, the ide...

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Thank you for submitting the enquiry. The customer is using an inappropriate secondary Ab. The primary Ab is raised in rabbit and so an anti rabbit secondary is necessary not anti goat! If you have any further questions then please do not hesitate to g...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"