Anti-alpha + beta Tubulin抗体[TU-10] (ab30506)

概述

  • 产品名称Anti-alpha + beta Tubulin抗体[TU-10]
  • 描述
    小鼠单克隆抗体[TU-10] to alpha + beta Tubulin
  • 特异性ab30506 recognizes alpha, beta-tubulin heterodimer, a basic intracellular structural unit of microtubules.
  • 经测试应用适用于: WB, ICCmore details
  • 种属反应性
    Reacts with a wide range of species. Not yet tested in other species.
  • 免疫原

    Microtubule proteins from porcine brain

  • 阳性对照
    • Porcine brain lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by precipitation methods and size-exclusion chromatography.
  • 克隆单克隆
  • 克隆编号TU-10
  • 同种型IgM
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab30506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 100 kDa. Use under reducing conditions.
ICC Use at an assay dependent dilution.

靶标

  • 相关性Tubulin is the major building block of microtubules - an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides alpha and beta. Alpha- and beta-tubulins are encoded by separate genes, or small gene families. Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 25 nm diameter. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions, the most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta tubulins consist of various isotypes and both undergo different post-translational modifications. Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape.
  • 细胞定位Cytoplasmic
  • 别名
    • TUBA1A antibody
    • TUBA3 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2A antibody
    • Tubulin alpha 1a antibody
    • Tubulin beta 2A antibody
    • Tubulin beta antibody
    see all

Anti-alpha + beta Tubulin antibody [TU-10] 图像

  • Anti-alpha + beta Tubulin antibody [TU-10] (ab30506) at 2 µg/ml + Human Jurkat T cell line lysate (1% laurylmaltoside)

    Secondary
    Goat anti-mouse IgG (H+L)-HRP

    Performed under non-reducing conditions.

    Predicted band size : 100 kDa

Anti-alpha + beta Tubulin antibody [TU-10] (ab30506)参考文献

ab30506 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"