Anti-Alpha B Crystallin (phospho S59)抗体(ab5577)

概述

  • 产品名称Anti-Alpha B Crystallin (phospho S59)抗体
    参阅全部 Alpha B Crystallin 一抗
  • 描述
    兔多克隆抗体to Alpha B Crystallin (phospho S59)
  • 经测试应用适用于: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
    预测可用于: Sheep, Rabbit, Hamster, Pig, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide corresponding to Human Alpha B Crystallin aa 54-64 (phospho S59).
    Sequence:

    FLRAPSWIDTG

  • 阳性对照
    • U373 MG cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab5577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 21126175
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 20 kDa.
ICC/IF Use a concentration of 8 µg/ml.
IHC-P Use a concentration of 8 µg/ml.

靶标

  • 功能May contribute to the transparency and refractive index of the lens.
  • 组织特异性Lens as well as other tissues.
  • 疾病相关Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
  • 序列相似性Belongs to the small heat shock protein (HSP20) family.
  • 细胞定位Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AACRYA antibody
    • Alpha B crystallin antibody
    • Alpha crystallin B chain antibody
    • Alpha(B)-crystallin antibody
    • Alpha-crystallin B chain antibody
    • CRYA2 antibody
    • Cryab antibody
    • CRYAB_HUMAN antibody
    • Crystallin alpha B antibody
    • Crystallin alpha polypeptide 2 antibody
    • CTPP2 antibody
    • Heat shock 20 kD like protein antibody
    • Heat shock protein beta 5 antibody
    • Heat shock protein beta-5 antibody
    • HspB5 antibody
    • Renal carcinoma antigen NY REN 27 antibody
    • Renal carcinoma antigen NY-REN-27 antibody
    • Rosenthal fiber component antibody
    see all

Anti-Alpha B Crystallin (phospho S59) antibody 图像

  • ab5577 at 0.5µg/ml concentration staining approximately 20 KDa Crystallin Alpha B in lane 1) phospho-alpha-B crystallin (Ser59) and lane 2) non phosphorylated alpha-B crystallin in U373 cell lysate.

  • ab5577 staining of (top panel) human kidney tissue sections, highlighting highly localised staining in tubular epithelial cells, and (bottom panel) human ovarian carcinoma sections. Formalin-fixed paraffin embedded sections of human kidney and ovarian carcinoma tissue were incubated with ab5577 (1/1000) for 2 hours at room temperature. Antigen retrieval was performed by heat induction in citrate buffer, pH 6.

    See Abreview

Anti-Alpha B Crystallin (phospho S59) antibody (ab5577)参考文献

This product has been referenced in:
  • Dammer EB  et al. Quantitative phosphoproteomics of Alzheimer's disease reveals cross-talk between kinases and small heat shock proteins. Proteomics 15:508-19 (2015). WB ; Human . Read more (PubMed: 25332170) »
  • Mitra A  et al. Cardioprotective role of P38 MAPK during myocardial infarction via parallel activation of a-crystallin B and Nrf2. J Cell Physiol 229:1272-82 (2014). Read more (PubMed: 24464634) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (adult kidney)
Specification adult kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Oct 21 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (kidney and ovarian carcinoma)
Specification kidney and ovarian carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid 10mM pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Apr 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Specification kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid 10mM pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

提交于 Apr 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"