This antibody gave a positive signal in Human Spinal Cord tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 20 kDa).
Use a concentration of 5 µg/ml.
May contribute to the transparency and refractive index of the lens.
Lens as well as other tissues.
Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.
Belongs to the small heat shock protein (HSP20) family.
Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Western blot - Anti-Alpha B Crystallin (phospho S59) antibody (ab103282)
All lanes : Anti-Alpha B Crystallin (phospho S59) antibody (ab103282) at 1 µg/ml
Lane 1 : Human spinal cord tissue lysate - total protein (ab29188) Lane 2 : Human spinal cord tissue lysate - total protein (ab29188) with Immunising peptide at 1 µg/ml Lane 3 : Human spinal cord tissue lysate - total protein (ab29188) with Non modified peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Alpha B Crystallin contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin (phospho S59) antibody (ab103282)
ab103282 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103282 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.