Anti-Alpha B Crystallin (phospho S19)抗体(ab5597)

概述

  • 产品名称
    Anti-Alpha B Crystallin (phospho S19)抗体
    参阅全部 Alpha B Crystallin 一抗
  • 描述
    兔多克隆抗体to Alpha B Crystallin (phospho S19)
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
    预测可用于: Sheep, Rabbit, Hamster, Pig, Cynomolgus monkey
  • 免疫原

    Synthetic peptide corresponding to Human Alpha B Crystallin aa 14-25.
    Sequence: FFPFHSpPSRLFD

  • 阳性对照
    • Immunofluorescence staining of phospho-alpha-B crystallin (Ser19) in U373 MG cells results in cytoplasmic staining.

性能

应用

Our Abpromise guarantee covers the use of ab5597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 20 kDa.

靶标

图片

  • All lanes : Anti-Alpha B Crystallin (phospho S19) antibody (ab5597) at 0.5 µg/ml

    Lane 1 : phospho-alpha-B crystallin (Ser19)
    Lane 2 : unphosphorylated alpha-B crystallin

    Observed band size: 20 kDa (why is the actual band size different from the predicted?)



    Western blot of recombinant bovine phospho-alpha-B crystallin (Ser19) using ab5597. Lane 1 is phospho-alpha-B crystallin (Ser19) and lane 2 is unphosphorylated alpha-B crystallin.

文献

This product has been referenced in:
  • Lambrecht S  et al. Differential expression of alphaB-crystallin and evidence of its role as a mediator of matrix gene expression in osteoarthritis. Arthritis Rheum 60:179-88 (2009). WB ; Human . Read more (PubMed: 19116904) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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