RabMAb

Anti-alpha Adducin (phospho S59)抗体[EP2175AY] (ab76251)

概述

  • 产品名称Anti-alpha Adducin (phospho S59)抗体[EP2175AY]
    参阅全部 alpha Adducin 一抗
  • 描述
    兔单克隆抗体[EP2175AY] to alpha Adducin (phospho S59)
  • 特异性ab76251 only detects alpha Adducin when phosphorylated at Serine 59.
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phospho-peptide corresponding to residues surrounding Serine59 of human alpha Adducin

  • 阳性对照
    • HeLa cell lysates, untreated, or treated with calyculin A
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab76251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: 1/40.
    WB: 1/2000 - 1/5000. Predicted molecular weight: 81 kDa.
    Is unsuitable for Flow Cyt, ICC and IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
    • 组织特异性Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.
    • 序列相似性Belongs to the aldolase class II family. Adducin subfamily.
    • 结构域Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.
    • 细胞定位Cytoplasm > cytoskeleton. Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ADD A antibody
      • ADD1 antibody
      • ADDA antibody
      • ADDA_HUMAN antibody
      • Adducin 1 (alpha) antibody
      • Alpha adducin antibody
      • Alpha-adducin antibody
      • Erythrocyte adducin alpha subunit antibody
      • Erythrocyte adducin subunit alpha antibody
      • MGC3339 antibody
      • MGC44427 antibody
      • OTTHUMP00000151163 antibody
      • OTTHUMP00000151164 antibody
      • OTTHUMP00000151165 antibody
      • OTTHUMP00000218664 antibody
      • OTTHUMP00000218665 antibody
      see all

    Anti-alpha Adducin (phospho S59) antibody [EP2175AY] 图像

    • All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate, treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP labelled IgG at 1/1000 dilution

      Predicted band size : 81 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)

    Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)参考文献

    ab76251 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76251.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"