重组Anti-alpha Adducin (phospho S59)抗体[EP2175AY] (ab76251)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-alpha Adducin (phospho S59)抗体[EP2175AY]
参阅全部 alpha Adducin 一抗 -
描述
兔单克隆抗体[EP2175AY] to alpha Adducin (phospho S59) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cell lysates, untreated, or treated with calyculin A
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP2175AY -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76251于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/5000. Predicted molecular weight: 81 kDa.
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说明 |
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WB
1/2000 - 1/5000. Predicted molecular weight: 81 kDa. |
靶标
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功能
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
组织特异性
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
序列相似性
Belongs to the aldolase class II family. Adducin subfamily. -
结构域
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
细胞定位
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 118 Human
- Omim: 102680 Human
- SwissProt: P35611 Human
- Unigene: 183706 Human
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别名
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
图片
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All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP labelled IgG at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab76251 被引用在 1 文献中.
- Bouyer G et al. Plasmodiumfalciparum infection induces dynamic changes in the erythrocyte phospho-proteome. Blood Cells Mol Dis 58:35-44 (2016). PubMed: 27067487