Anti-alpha Adducin (phospho S726)抗体[EP734Y] (ab40760)
Key features and details
- Rabbit monoclonal [EP734Y] to alpha Adducin (phospho S726)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-alpha Adducin (phospho S726)抗体[EP734Y]
参阅全部 alpha Adducin 一抗 -
描述
兔单克隆抗体[EP734Y] to alpha Adducin (phospho S726) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa cell lysate. IHC-P: Human lung tissue. ICC/IF: HeLa cells.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP734Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab40760于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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说明 |
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WB
1/5000 - 1/50000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
靶标
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功能
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
组织特异性
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
序列相似性
Belongs to the aldolase class II family. Adducin subfamily. -
结构域
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
细胞定位
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 118 Human
- Entrez Gene: 11518 Mouse
- Entrez Gene: 24170 Rat
- Omim: 102680 Human
- SwissProt: P35611 Human
- SwissProt: Q9QYC0 Mouse
- SwissProt: Q63028 Rat
- Unigene: 183706 Human
see all -
别名
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
图片
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All lanes : Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate 20ug, then the membrane was treated with alkaline phosphatase for 1 hour
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 120 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 7 secondsBlocking and diluting buffer: 5% NFDM/TBST
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Anti-alpha Adducin (phospho S726) antibody [EP734Y] (ab40760) at 1/50000 dilution + HeLa cell lysate at 10 µg
Predicted band size: 120 kDa
Observed band size: 120 kDa -
ab40760 (1/250), staining human lung by immunohistochemistry, paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab40760, (1/250) staining HeLa cells by immunofluorescence.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab40760 被引用在 2 文献中.
- Sheu SH et al. A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility. Cell 185:3390-3407.e18 (2022). PubMed: 36055200
- Thauland TJ et al. The Actin-Capping Protein Alpha-Adducin Is Required for T-Cell Costimulation. Front Immunol 10:2706 (2019). PubMed: 31824498