Other Immunogen Type corresponding to alpha Adaptin. Purified adaptors.
Our Abpromise guarantee covers the use of ab2730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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ICC/IF | Use a concentration of 5 µg/ml. | |
ICC | Use at an assay dependent concentration. | |
Inhibition Assay | Use at an assay dependent concentration. | |
Electron Microscopy | Use at an assay dependent concentration. PubMed: 23372794 | |
ELISA | Use at an assay dependent concentration. | |
Blocking | Use at an assay dependent concentration. | |
IP | Use at an assay dependent concentration. | |
WB | 1/1000. This antibody detects an ~100 kDa doublet representing the two isoforms of alpha-adaptin in rat brain, though alpha C is the dominant form seen. Microinjection of cells with this antibody results in variable blockade of endocytosis. | |
IHC-FoFr | Use at an assay dependent concentration. | |
Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Overlay histogram showing MCF7 cells stained with ab2730 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2730, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"