Anti-alpha Actinin抗体[MAC 276] (ab50599)

概述

  • 产品名称Anti-alpha Actinin抗体[MAC 276]
    参阅全部 alpha Actinin 一抗
  • 描述
    大鼠单克隆抗体[MAC 276] to alpha Actinin
  • 特异性Antibody reacts with a-actinin in Lethocerus and Drosophila flight and non-flight muscle. Antibody also reacts with rat skeletal muscle, human cardiac muscle, chicken gizzard muscle and human fibroblasts.
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-P, Electron Microscopy, WBmore details
  • 种属反应性
    与反应: Rat, Chicken, Human, Drosophila melanogaster, Lethocerus indicus (Waterbug)
  • 免疫原

    Flight muscle extract from Lethocerus indicus (Waterbug)

  • 阳性对照
    • In Western Blot, this antibody gave a positive signal in human and rat skeletal muscle tissue lysates, and in rat heart tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal bladder.

性能

应用

Our Abpromise guarantee covers the use of ab50599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Electron Microscopy Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).

靶标

  • 功能F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • 序列相似性Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • actinin 1 smooth muscle antibody
    • Actinin alpha 1 antibody
    • actinin, alpha 1 antibody
    • ACTN 1 antibody
    • Actn1 antibody
    • ACTN1_HUMAN antibody
    • Alpha Actinin 1 antibody
    • Alpha actinin cytoskeletal isoform antibody
    • Alpha-actinin cytoskeletal isoform antibody
    • Alpha-actinin-1 antibody
    • BDPLT15 antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    • FLJ40884 antibody
    • FLJ54432 antibody
    • Non muscle alpha actinin 1 antibody
    • Non-muscle alpha-actinin-1 antibody
    see all

Anti-alpha Actinin antibody [MAC 276] 图像

  • IHC image of alpha Actin staining in Human normal bladder formalin fixed paraffin embedded tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins at 98°C. The section was incubated with ab50599, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

  • ICC/IF image of ab68826 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab50599 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab50599 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50599, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • All lanes : Anti-alpha Actinin antibody [MAC 276] (ab50599) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Heart (Rat) Tissue Lysate
    Lane 3 : Skeletal Muscle (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 103 kDa
    Observed band size : 103 kDa


    Exposure time : 8 minutes

Anti-alpha Actinin antibody [MAC 276] (ab50599)参考文献

This product has been referenced in:
  • Sehring IM  et al. An equatorial contractile mechanism drives cell elongation but not cell division. PLoS Biol 12:e1001781 (2014). ICC/IF ; Sea Tunicate . Read more (PubMed: 24503569) »
  • Chechenova MB  et al. The Drosophila Z-disc protein Z(210) is an adult muscle isoform of Zasp52, which is required for normal myofibril organization in indirect flight muscles. J Biol Chem 288:3718-26 (2013). IHC-Fr ; Drosophila melanogaster . Read more (PubMed: 23271733) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Cell (cardiomyocytes)
Specification cardiomyocytes
Permeabilization Yes - Triton X-100
Fixative Methanol
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提交于 Jun 23 2014

The guarantee is a promise of a replacement, credit, or refund if an antibody fails in an application listed on the datasheet, with samples derived from the species listed in the "Cross-reactivity" field of the datasheet. For ab2787, as an example, the...

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Thank you for sending the fruit fly sequences. Alignment of the following two sequences with the immunogens fo the antibodies we have against the human targets did not reveal any significant homology, (i.e., none greater than 50% identity) and I am ske...

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Please find below the discount code we discussed for ab50599. Please send the Drosophila SQSTM1 / p62 sequence at your convenience and I will align it with the ab56416 immunogen sequence.

I am very pleased to hear you would like to accept ou...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Cardiomyocytes and fibroblasts (from ES cell diff))
Specification Cardiomyocytes and fibroblasts (from ES cell diff)
Fixative Methanol
Permeabilization Yes - 0.1% Triton-X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"