概述

  • 产品名称Anti-alpha Actinin 4抗体
    参阅全部 alpha Actinin 4 一抗
  • 描述
    兔多克隆抗体to alpha Actinin 4
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 206-512 of Human alpha Actinin 4 (AAH05033).

  • 阳性对照
    • 293T, A431, H1299, HepG2 whole cell lysates HeLa cells; A549 xenograft (IHC)

性能

应用

Our Abpromise guarantee covers the use of ab96686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 105 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
  • 组织特异性Widely expressed.
  • 疾病相关Defects in ACTN4 are the cause of focal segmental glomerulosclerosis type 1 (FSGS1) [MIM:603278]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • 序列相似性Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • 细胞定位Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines.
  • Information by UniProt
  • 数据库链接
  • 别名
    • actinin 4 antibody
    • Actinin alpha 4 antibody
    • actinin4 antibody
    • ACTN 4 antibody
    • ACTN4 antibody
    • ACTN4_HUMAN antibody
    • Alpha-actinin-4 antibody
    • DKFZp686K23158 antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    • Focal segmental glomerulosclerosis 1 antibody
    • FSGS 1 antibody
    • FSGS antibody
    • FSGS1 antibody
    • Non muscle alpha actinin 4 antibody
    • Non-muscle alpha-actinin 4 antibody
    see all

Anti-alpha Actinin 4 antibody 图像

  • All lanes : Anti-alpha Actinin 4 antibody (ab96686) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 105 kDa
  • ab96686, at 1/200 dilution, staining names alpha Actinin 4 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
  • ab96686, at 1/500 dilution, staining alpha Actinin 4 in paraffin-embedded A549 xenograft by Immunohistochemistry.

Anti-alpha Actinin 4 antibody (ab96686)参考文献

This product has been referenced in:
  • Kalendová A  et al. Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block. Histochem Cell Biol 142:139-52 (2014). Read more (PubMed: 25002125) »

See 1 Publication for this product

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