概述

  • 产品名称
    Anti-alpha A Crystallin抗体[c9F2]
    参阅全部 alpha A Crystallin 一抗
  • 描述
    小鼠单克隆抗体[c9F2] to alpha A Crystallin
  • 经测试应用
    适用于: Blocking, Flow Cyt, Sandwich ELISA, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein (Human).

性能

应用

Our Abpromise guarantee covers the use of ab14821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Blocking Use at an assay dependent concentration. PubMed: 22359280
Flow Cyt Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration. PubMed: 22359280
IHC-P Use at an assay dependent concentration. PubMed: 22359280
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 20 kDa.

靶标

  • 功能
    May contribute to the transparency and refractive index of the lens.
  • 疾病相关
    Defects in CRYAA are a cause of cataract autosomal dominant (ADC) [MIM:604219]. Cataract is an opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Cataract is the most common treatable cause of visual disability in childhood.
  • 序列相似性
    Belongs to the small heat shock protein (HSP20) family.
  • 翻译后修饰
    O-glycosylated; contains N-acetylglucosamine side chains.
    Deamidation of Asn-101 in lens occurs mostly during the first 30 years of age, followed by a small additional amount of deamidation (approximately 5%) during the next approximately 38 years, resulting in a maximum of approximately 50% deamidation during the lifetime of the individual.
    Phosphorylation on Ser-122 seems to be developmentally regulated. Absent in the first months of life, it appears during the first 12 years of human lifetime. The relative amount of phosphorylated form versus unphosphorylated form does not change over the lifetime of the individual.
  • 细胞定位
    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acry 1 antibody
    • Alpha crystallin A chain antibody
    • Alpha-crystallin A chain antibody
    • CRYA 1 antibody
    • CRYA1 antibody
    • CRYAA antibody
    • CRYAA_HUMAN antibody
    • Crystallin alpha 1 antibody
    • Crystallin alpha A antibody
    • Heat shock protein beta 4 antibody
    • Heat shock protein beta-4 antibody
    • HSPB 4 antibody
    • HspB4 antibody
    • short form antibody
    • Zonular Central Nuclear Cataract antibody
    see all

图片

  • Flow Cytometry analysis of Balb/3T3 cells labelling CRYAA with ab14821, staining at 2-5 µg/1x106 cells (red). A 488-conjugated goat anti-mouse IgG was used as the secondary antibody. Black - Isotype control, mouse IgG.

文献

This product has been referenced in:
  • Fan Q  et al. Identification of proteins that interact with alpha A-crystallin using a human proteome microarray. Mol Vis 20:117-24 (2014). WB ; Human . Read more (PubMed: 24453475) »
  • Oh JY  et al. Identification of the HSPB4/TLR2/NF-?B axis in macrophage as a therapeutic target for sterile inflammation of the cornea. EMBO Mol Med 4:435-48 (2012). WB, IHC-P, Blocking, Sandwich ELISA ; Mouse, Rat . Read more (PubMed: 22359280) »

See all 2 Publications for this product

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