Anti-Alpha A + alpha B Crystallin抗体(ab28163)

概述

  • 产品名称Anti-Alpha A + alpha B Crystallin抗体
    参阅全部 Alpha A + alpha B Crystallin 一抗
  • 描述
    兔多克隆抗体to Alpha A + alpha B Crystallin
  • 特异性ab28163 recognises alpha A and alpha B crystallin proteins. There is no reactivity with beta crystallin.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 免疫原

    Synthetic peptides:

    EEKPTSAPSS

    conjugated to KLH, corresponding to amino acids 164/173 of Human alpha A; and REEKPAVTAAPKK conjugated to KLH, corresponding to amino acids 163/175 of Human alpha B Crystallin

  • 阳性对照
    • Bovine alpha Crystallin Protein, Bovine alpha A Crystallin Protein, Bovine alpha B Crystallin Protein

性能

应用

Our Abpromise guarantee covers the use of ab28163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: 1/1000. This dilution was sufficient for detection of alpha A/beta B-crystallin in 20µg of alpha A-crystallin protein by colorimetric immunoblot analysis Goat anti-Rabbit IgG-AP as secondary antibody. Predicted molecular weight: 20 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Crystallins are separated into two classes: taxon specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Alpha crystallins are composed of two gene products: alpha A and alpha B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture.
    • 细胞定位Cytoplasmic
    • 数据库链接
    • 别名
      • Alpha crystallin A chain antibody
      • Alpha crystallin B chain antibody
      • CRYA1 antibody
      • CRYA2 antibody
      • CRYAA antibody
      • CRYAB antibody
      • Heat shock protein beta 4 antibody
      • Heat shock protein beta 5 antibody
      • HspB4 antibody
      • HspB5 antibody
      • Rosenthal fiber component antibody
      see all

    Anti-Alpha A + alpha B Crystallin antibody 图像

    • All lanes : Anti-Alpha A + alpha B Crystallin antibody (ab28163) at 1/1000 dilution

      Lane 1 : Tissue lysates prepared from mouse heart
      Lane 2 : Cell lysates prepared from Hela cells
      Lane 3 : Crystallin alpha B native bovine protein
      Lane 4 : Crystallin alpha native bovine protein
      Lane 5 : Molecular weight marker


      Predicted band size : 20 kDa

    Anti-Alpha A + alpha B Crystallin antibody (ab28163)参考文献

    ab28163 has not yet been referenced specifically in any publications.

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