Anti-alpha Tubulin (phospho Y272)抗体[EP1334(2)Y] (ab76290)
Key features and details
- Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
概述
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产品名称
Anti-alpha Tubulin (phospho Y272)抗体[EP1334(2)Y]
参阅全部 alpha Tubulin 一抗 -
描述
兔单克隆抗体[EP1334(2)Y] to alpha Tubulin (phospho Y272) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat cell lysates treated with pervanadate
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP1334(2)Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/25000 - 1/50000. Predicted molecular weight: 54 kDa.
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说明 |
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WB
1/25000 - 1/50000. Predicted molecular weight: 54 kDa. |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 7277 Human
- Omim: 191110 Human
- SwissProt: P68366 Human
- Unigene: 75318 Human
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别名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
图片
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All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution
Lane 1 : Jurkat cell lysates, untreated.
Lane 2 : Jurkat cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 54 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab76290 被引用在 3 文献中.
- Paech F et al. Hepatocellular Toxicity Associated with Tyrosine Kinase Inhibitors: Mitochondrial Damage and Inhibition of Glycolysis. Front Pharmacol 8:367 (2017). WB ; Human . PubMed: 28659801
- Yu Y et al. Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules. Cancer Cell 28:82-96 (2015). PubMed: 26096845
- Meshki J et al. Substance P induces rapid and transient membrane blebbing in U373MG cells in a p21-activated kinase-dependent manner. PLoS One 6:e25332 (2011). WB . PubMed: 21966499