Application
Western blot
Sample
Mouse Tissue lysate - whole (forebrain)
Loading amount
10 µg
Specification
forebrain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Concentration
0.5 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk TBS tween 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: sheep anti mouse hrp amsersham
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL plus
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: ~55 kDa
Additional data
Additional Notes
slight smearing below the band, but otherwise no problems - 1 band
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 19 2007