Application
Western blot
Sample
Plants Tissue lysate - whole (Nicotiana benthamiana)
Gel Running Conditions
Non-reduced Denaturing (4-20% precut gels)
Loading amount
10 µg
Treatment
lysis buffer 12h
Specification
Nicotiana benthamiana
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Additional data
Additional Notes
the tubulin should have a band a little bit higher than 5okd, however, no band showed at that position. With the same process, I got very clear GFP band (~27kd).
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback.
As the antibody had not yet been tested with this sample type before, we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Huan Zhang
Verified customer
提交于 Nov 13 2017