Application
Western blot
Sample
Human Cell lysate - whole cell (Hek 293)
Loading amount
10 µg
Specification
Hek 293
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Other product details
Dilution
1/1500
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1:1 PBS/Licor Blocking Buffer
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Mouse IgG (H + L)
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Odyssey Infrared Imager
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55 kDa
Positive control
HEK 293 Cell Lysate Lane 4
Negative control
Immunoprecipitation with beads only (no ab for suspected binding partner)Lane 3
Additional data
Additional Notes
Lane 1 MW Ladder in kDa (from top: 118, 90, 50, 34, 26, 19). Lanes 2-4 - IPs of HEK293 cells transfected with suspected tubulin binding partner (2 & 4), empty vector cDNAs (3). Lane 4 did not have any antibody specific to the binding partner. Lane 5 - HEK 293 cell lysate.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Chris Tracy
Verified customer
提交于 Jan 12 2012