Application
Immunohistochemistry (Frozen sections)
Sample
Xenopus laevis Tissue sections (stage 36, transverse cryosection, Xenopus laevis)
Specification
stage 36, transverse cryosection, Xenopus laevis
Fixative
MEMFa
Permeabilization
No
Blocking step
BSA/Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 2%BSA / 2%Serum / PBS
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
FITC-tubulin shown in green highlights neurons in neural tube and ciliated epidermal cells. DAPI is in grey. Laminin (abcam 11575) is in blue (donkey anti-rabbit Cy5 secondary). E-cadherin is in red (donkey anti-mouse Cy3 secondary).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 11 2010