Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% milk/TBS
Dilution
1/1000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL+
Exposure
20 minute(s) and 0 second(s)
Positive control
10 samples in which other antigens had been previously successfully detected on the same blot
Additional data
Additional Notes
This antibody has never successfully detected any bands, over a number of attempts. It 100% does not work for WB applications.
Abcam response
We are sorry to see you had trouble with this antibody. The expiration date of the lot you have is September 2012 and we suspect either the HRP has lost activity or the antibody has lost affinity since then.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 12 2015