Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2c12)
Loading amount
30 µg
Specification
C2c12
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/2500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
10 second(s)
Bands
Specific: 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 18 2010