Anti-Alpha-synuclein (phospho S129)抗体(ab42906)
Key features and details
- Rabbit polyclonal to Alpha-synuclein (phospho S129)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Alpha-synuclein (phospho S129)抗体
参阅全部 Alpha-synuclein 一抗 -
描述
兔多克隆抗体to Alpha-synuclein (phospho S129) -
宿主
Rabbit -
特异性
This antibody is specific for the 15 kDa alpha Synuclein protein phosphorylated at serine 129. Immunolabelling is blocked by the phosphopeptide used as an antigen but not by the corresponding dephosphopeptide. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat
预测可用于: Mouse, Cow, Dog, Non human primates -
免疫原
Synthetic peptide corresponding to Rat Alpha-synuclein (phospho S129). Phosphopeptide corresponding to amino acid residues surrounding the phospho serine 129 of Rat alpha Synuclein.
Database link: P37840 -
阳性对照
- Rat cortex lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Prepared from rabbit serum by affinity purification via sequential chromatography on phosph- and dephosphopeptide affinity columns. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab42906于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 15 kDa.
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说明 |
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WB
1/1000. Predicted molecular weight: 15 kDa. |
靶标
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功能
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
组织特异性
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
疾病相关
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
序列相似性
Belongs to the synuclein family. -
结构域
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
翻译后修饰
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
细胞定位
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 478478 Dog
- Entrez Gene: 20617 Mouse
- Entrez Gene: 29219 Rat
- SwissProt: O55042 Mouse
- SwissProt: P37377 Rat
- Unigene: 17484 Mouse
- Unigene: 1827 Rat
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别名
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
图片
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All lanes : Anti-Alpha-synuclein (phospho S129) antibody (ab42906) at 1/1000 dilution
Lane 1 : Rat cortex lysate at 20 µg
Lane 2 : Rat cortex lysate with immunogenic peptide
1ng
Predicted band size: 15 kDa
Western blot of rat cortex lysate showing specific labelling of the ~15kDa alpha Synuclein protein phosphorylated at serine 129, using ab42906 at a 1/1000 dilution. Immunolabeling is blocked by the phosphopeptide (peptide) used as immunogen.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab42906 被引用在 2 文献中.
- Toba S et al. Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport. Sci Rep 7:16386 (2017). PubMed: 29180624
- Stoica G et al. Potential role of a-synuclein in neurodegeneration: studies in a rat animal model. J Neurochem 122:812-22 (2012). PubMed: 22639889